Strain Name |
C57BL/6-Il2tm1(IL2)Bcgen Il2ratm1(IL2RA) Bcgen ll2rbtm2(IL2RB)Bcgen ll2rgtm2(IL2RG)Bcgen/Bcgen
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Common Name | B-hIL2/hIL2RA/hIL2RB/hIL2RG mice |
Background | C57BL/6 | Catalog number | 112748 |
Aliases |
IL2 also known as TCGF, lymphokine
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Protein expression analysis
Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice by flow cytometry. Splenocytes were collected from wild-type mice and homozygous B-hIL2/hIL2RA/hIL2RB mice, and analyzed by flow cytometry with the species-specific anti-IL2RA antibody. Human IL2RA was exclusively detectable in T cells, CD4+ T cells and Treg cells of homozygous B-hIL2/hIL2RA/hIL2RB mice but not in wild-type mice. Mouse IL2RA was only detectable in these cells of wild-type mice. However, IL2RA was not detectable in CD8+ T cells in both these two mice.
Strain specific IL2RB and IL2RG expression analysis in heterozygous and homozygous B-hIL2RB/hIL2RG mice by flow cytometry. Splenocytes were collected from wild-type mice and B-hIL2RB/hIL2RG mice, and analyzed by flow cytometry with species-specific antibody. Human IL2RB was detectable in heterozygous (H/+;H/+) and homozygous B-hIL2RB/hIL2RG mice (H/H;H/H). Human IL2RG was too low to be detectable in heterozygous, but detectable in homozygous B-hIL2RB/hIL2RG mice.
Intracellular phosphorylation of pSTAT5 analysis in splenocytes by flow cytometry. Splenocytes were harvested and analyzed for pSTAT5 induction in the T cell subtypes of wild type C57BL/6 mice and B-hIL2/hIL2R mice. IL2 induced pSTAT5 in T cells with a dose dependent manner. Specifically induced high level of pSTAT5 in CD8+T cells. At 10ng/mL concentration, human IL2 induced higher pSTAT5 in T cells of B-hIL2/hIL2R mice than that in wild type C57BL/6 mice.
Summary