Strain Name |
C57BL/6-Il12atm1(IL12A)BcgenIl12btm1(IL12B)BcgenIl12rb1tm1(IL12RB1)Bcgen Il12rb2tm1(IL12RB2)Bcgen/Bcgen |
Common Name | B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice |
Background | C57BL/6 | Catalog number | 140575 |
Related Genes |
IL12A also known as P35, CLMF, NFSK, NKSF1, IL-12A IL12B also known as CLMF, NKSF, CLMF2, IMD28, IMD29, NKSF2, IL-12B IL12RB1 also known as CD212, IMD30, IL12RB, IL-12R-BETA1 |
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NCBI Gene ID |
16159,16160,16161,16162 |
mRNA expression analysis
Protein expression analysis
Function analysis
IL12 induced the IFN-γ production in CD4+ T cells sorted from splenocytes. CD4+ T cells were sorted from the splenocytes in the wild-type (+/+) and homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (H/H) (n=3), the production of IFN-γ in supernatants were assessed after 48 h of incubation with 0.02 or 0.2 μg/mL rhIL-12 in combination with bead-associated CD3 and CD28 mAbs, under the condition in the panel. For comparison, with 0.01 or 0.05 μg/mL of rmIL-12 as control. Mouse IFN-γ were both increased after responsiveness to mIL-12 in wild-type mice and hIL-12 in homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (H/H). The humanized mice were successfully constructed.
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice were similar to those in the C57BL/6 mice, demonstrating that the humanization does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Blood chemistry tests of B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice. Serum from the C57BL/6 and B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (n=6, 7 week-old) was collected and analyzed for levels of indicators. The measurements of B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice were similar to that in C57BL/6 mice, indicating that humanization does not change the health of related tissues, such as liver. Values are expressed as mean ± SEM.
IL12 is a powerful partner in tumor immunotherapy
Antitumor activity of a modified human IL12 as an immune modulator in B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice bearing colon cancer MC38 cells model. (A) Modified human IL12 from the cooperation customer inhibited MC38 tumor growth. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (female, 7-9 week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with drugs in the panel. (B) Body weight changes during treatment. As shown in panel A, the modified human IL12 shows inhibitory effects but the tumor recovers growth when the drug was stopped. Values are expressed as mean ± SEM.