Frequency of leukocyte subpopulations in spleen

Comparison of body weights and tissues weights between WT and B-hIGF1R mice. The body weights of humanized mice were heavier than those of WT control mice regardless of gender(A). The weight of tissues such as the brain, spleen and kidney in female B-hIGF1R mice were heavier than those of WT female mice(C) while there is no difference in those three tissues’ weight between the male WT and B-hIGF1R mice(B). There is no difference in the weight of heart and lung between the male WT and B-hIGF1R mice regardless of gender.  The weights of liver in B-hIGF1R mice were heavier than those of WT mice regardless of gender.

Figitumumab induces IGF-1R internalization and down-regulation in B-hIGF1R mice

Western blot analysis of Phospho-IGF1R/AKT protein expression in human breast cancer MCF-7 cells and kidney cells from WT and homozygous B-hIGF1R mice. Kidney cells were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hIGF1R mice (H/H), and then cultured with Figitumumab (1 μg/ml) or/and recombinant human IGF1 protein (200 ng/ml). Finally, these cells were analyzed by western blot with anti-IGF1R antibody (CST, 3027S), anti-phospho-IGF1R antibody (CST, 3024S) and anti-phospho-AKT antibody (CST, 4060S). Total proteins were loaded for western blot analysis. The human IGF1R was detected in homozygous B-hIGF1R mice and MCF-7 cells. After the addition of the human IGF1, the phospho-IGF1R and phospho-AKT were detected in wild-type, homozygous B-hIGF1R mice and MCF-7 cells. This result indicated that the human IGF1 can activate the phosphorylation of IGF1R and AKT, and this phosphorylation can be inhibited by the positive drug Figitumumab.