Flow cytometry is a technology used for rapid screening and analysis of single cells in solution. A flow cytometer uses a laser as a light source to produce scattered light and fluorescence signals, which are detected by photodiodes or photomultiplier tubes. These signals are converted into electronic signals and output as standardized format (.fcs) data files. Specific cell subpopulations can be analyzed or purified based on their fluorescence or light scattering characteristics.

TILs Detection：

Leukocyte/T/B/NK/Treg/Macrophage/Myeloid/DC/MDSC/Eosinophils/Neutrophils/Basophils/Monocytes/Cancer Cell 
Sample type: Blood, cell, tumor
Experimental cycle: 1-2 work days
Report: 3-7 work days

Multi cytokine detection ：
Luminex/CBA
Sample type : Serum, cell culture supernatant, etc
Experimental cycle : 1-2 work days
Report : 2-3 work days
Quantitative expression of membrane proteins
Sample type: Cell
Experimental cycle : 1 work day
Report : 1-2 work days
Cell activity/apoptosis/proliferation/binding/blocking/toxicity ：
Checkpoint/Function/proliferation/ADCC/ADCP
Sample type : Cell
Experimental cycle : 1-2 work days
Report : 3-4 work days
Receptor Occupancy：
Sample type : Blood, cells
Experimental cycle : 1-2 work days
Report : 3-5 work days

Luminex technology is a multi-functional liquid-phase analysis platform developed based on colored microspheres, laser technology, fluidics, and high-speed digital signal processing. Its core is to encode polypropylene or magnetic microspheres with fluorescent dyes, and to obtain up to 100 microspheres with different fluorescence spectra by adjusting the ratio of two fluorescent dyes. Each type of microsphere is covalently cross-linked with capture antibodies directed against specific antigens. Up to 100 detection reactions can be simultaneously conducted in each reaction well. During operation, the instrument identifies the microsphere coding and fluorescence signal intensity of the reporting molecule on the microsphere by two lasers.

Technological advantages：High sensitivity and specificity, Good repeatability, Low sample size requirement, Short detection time, Customizable indicator combinations for project development.
Sample type： Serum, plasma, cell culture supernatant, tissue lysates and other body fluids (alveolar lavage fluid, urine, aqueous humor, saliva, tears, etc.).

Electrochemiluminescence (ECL)

Based on the immunoassay technology of Electrochemiluminescence (ECL) method, the HRP used in the classical ELISA for color reaction is replaced by Ru (bipyridyl), and the circuit is formed by the graphite electrode immune reaction system. The process of making the trivalent ruthenium metal lose electrons to become divalent ruthenium can emit light of 620 nm wavelength, and the amount of the substance to be measured can be distinguished by light intensity. In practical application, it can obtain a wider linear range and higher sensitivity than ELISA method. Compared with ELISA, it has higher sensitivity: 1~4 ng/mL, wider linear range: 3~5 logs, less serum sample size, more sampling time points that can be designed on the same mouse, and more accurate analysis of pharmacokinetic parameters.

IncuCyte is a set of live cell imaging and analysis platform for long-time dynamic, non injurious. Researchers can observe cell behavior in real time, and it can conduct long-term quantitative analysis of cell proliferation, migration and morphology within days or weeks. The system uses bright field and fluorescence imaging to capture high-resolution images at up to 20x magnification. Images can be automatically analyzed using IncuCyte software to quickly collect data and quantify cells in culture.

IncuCyte S3 live-cell analysis system

Pharmacokinetics (PK) is an important part of drug development, studying the changes in drug concentration over time in the body, including absorption, distribution, metabolism, and excretion. Preclinical PK studies explore the characteristics and transformation process of drugs/metabolites in vivo and in vitro, and establish dose-response relationships as a reference for new drug development and clinical trials. We have extensive experience in preclinical pharmacokinetic testing and bioanalysis, providing professional PK analysis services for antibody drugs. Although PK studies of antibodies differ greatly from traditional chemical drugs, they are still based on classical pharmacology research methods and focus more on the pharmacological properties affected by antibody targeting and properties. PK includes two types of experiments: the detection of antibody serum/plasma concentration and the screening of anti-drug antibodies (ADA). The PK bioanalytical platform includes ELISA and ECL.

Antibody serum/plasma concentration detection (general method)
Sample type: animal/human serum/plasma
Experimental period: 3-4 work days
Report period: 2-3 work days

Specific PK/anti-drug antibody (ADA) screening test
Sample type: animal/human serum/plasma
Method development and validation period: 3-4 work days
Experimental period: 3-4 work days
Report period: 2-3 work days
General method :  Coated with conventional sheep anti-human IgG heavy chain/light chain for detection.
Specific method :  Coating with the specific antigen of the sample being tested requires method development and validation.
Advice : I.V. administration, PK testing with serum, 9-10 week-old mice, EDTA anticoagulants (EDTA.2Na and EDTA.2K)

ELISpot is a sensitive assay for quantifying analyte secreting cells at the single-cell level. Cytokines, immunoglobulins or other target proteins secreted by cells are captured by specific antibodies immediately after secretion and throughout the stimulation process. The detection level of ELISpot is as low as one in 2.5×105  cells, and it is one of the most sensitive cell detection methods.

Principle

Advantages
High sensitivity, which can detect the secretion frequency of cytokines or immunoglobulins at the single-cell level
High specificity, capable of functional detection of live cells after antigen stimulation

Sample Type：Cell（2 x 10⁵cells / well）
Experimental cycle：3-5 work days
Report：2-3 work days