ESC-Based Gene Targeting

Embryonic stem cell or ESC-based gene targeting uses a stem cell's plasticity to engineer gene-targeted mice. Mouse stem cells are first electroporated with a targeting vector. Following successful homologous recombination, targeted embryonic stem cells are then injected into a normal mouse blastocyst. When injected, the stem cells colonize the blastocyst, resulting in a chimera with cells of distinct genotypes. The blastocysts are then transplanted into surrogate mice. Finally, chimeric mice are bred with wild mice strains to obtain model animals that have germline transmission of the targeted gene.

Knockout Mouse Models

In ESC-based gene knockout targeting, we use a positive selection marker to replace an exon of a targeted gene. The targeted gene is deleted globally, whereas in conditional knockout animals, the gene is deleted only in selected cells, as determined by a tissue specific promoter. Conditional knockout mouse models are usually generated using Cre-LoxP or Flp-Frt recombination systems.

Knockin Mouse Models

To generate a knockin mouse, a DNA fragment of interest is inserted at the desired location in the genome. This modality also allows for a variety of models to be created including point mutant, reporter (GFP), tagged (FLAG), Cre, and humanized mice.

Biocytogen Gene-Edited Animal/Cell Line Models

Since its inception in 2008, Biocytogen has completed more than 2,500 gene editing projects, providing gene-edited rat and mouse models for many academic and industrial studies. We aim to accelerate the generation of client models by improving the HR efficiency by 10-20 times via innovative EGE technology development.

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