B-hIL2/hIL2RA/hIL2RB/hIL2RG mice
| Strain Name |
C57BL/6-Il2tm1(IL2)Bcgen Il2ratm1(IL2RA) Bcgen ll2rbtm2(IL2RB)Bcgen ll2rgtm2(IL2RG)Bcgen/Bcgen
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Common Name | B-hIL2/hIL2RA/hIL2RB/hIL2RG mice |
| Background | C57BL/6 | Catalog number | 112748 |
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Aliases |
IL2 also known as TCGF, lymphokine
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Model validation
Protein expression analysis
Strain specific IL2 expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by ELISA. Serum were collected from wild-type mice C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-mCD3e antibody (7.5 μg/mice, i.p.) and anti-CD28 antibody (5 μg/mice, i.p.) in vivo for 2 h, and analyzed by ELISA with species-specific IL2 ELISA kit. (A) Mouse IL2 was only detectable in wild-type mice. (B) Human IL2 was exclusively detectable in homozygous mice but not in wild-type mice.
Mice used in the experiment: male, 6-week-old, n=3/group.
Protein expression analysis of IL2RA in T cells
Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. mIL2RA was only detectable in wild-type mice, and hIL2RA was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, n=3/group.
Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. mIL2RA was only detectable in wild-type mice, and hIL2RA was exclusively detectable in CD4+ T and CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, n=3/group.
Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RB antibody. mIL2RB was only detectable in wild-type mice, and hIL2RB was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, n=3/group.
Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice stimulated with anti-CD3e antibody in vivo (7.5 μg/mice, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RB antibody. mIL2RB was only detectable in wild-type mice, and hIL2RB was exclusively detectable in CD4+ T and CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, n=3/group.
Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, 1 mice/group.
Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in CD4+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, 1 mice/group.
Strain specific IL2RG expression analysis in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice, and cultured in 96-well plates pre-coated with 2 μg/mL anti-mCD3e and 5 μg/mL anti-mCD28 antibody for 24 h, and then cells were harvested and analyzed by flow cytometry with species-specific anti-IL2RG antibody. mIL2RG was only detectable in wild-type mice, while hIL2RG was exclusively detectable in CD8+ T cells in homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice.
Mice used in the experiment: male, 8-week-old, 1 mice/group.
Intracellular phosphorylation of pSTAT5 analysis in splenocytes by flow cytometry. Splenocytes were harvested and analyzed for pSTAT5 induction in the T cell subtypes of wild type C57BL/6 mice and B-hIL2/hIL2R mice. IL2 induced pSTAT5 in T cells with a dose dependent manner. Specifically induced high level of pSTAT5 in CD8+T cells. At 10ng/mL concentration, human IL2 induced higher pSTAT5 in T cells of B-hIL2/hIL2R mice than that in wild type C57BL/6 mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, dendritic cells, neutrophils, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells and monocytes decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, dendritic cells, neutrophils, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells and monocytes decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in lymph nodes by flow cytometry. Lymph nodes cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. NK cells decreased in humanized mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in thymus by flow cytometry. Thymus cells were isolated from male wild-type C57BL/6 mice (n=3, 8-week-old) and homozygous B-hIL2/hIL2RA/IL2RB/IL2RG mice (n=3, 9-week-old). A. Flow cytometry analysis of the thymus cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, CD4+ T cells, CD8+ T cells and Tregs in B-hIL2/hIL2RA/IL2RB/IL2RG mice were similar to those in C57BL/6 mice. Values are expressed as mean ± SD. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
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