mRNA expression analysis

mRNA expression profile of IL36R

IHC analysis of IL36R expression

Immunohistochemical (IHC) analysis of IL36R expression in homozygous B-hIL36R mice. 

Analysis of spleen T cell subpopulations in B-hIL36R mice

Analysis of lymph node leukocyte subpopulations by FACS.

Analysis of blood leukocytes cell subpopulations in B-hIL36R mice

Analysis of blood leukocyte subpopulations by FACS.Blood cells were isolated from female C57BL/6 and B-hIL36R mice (n=3, 7 week-old). Flow cytometry analysis of the blood leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hIL36R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL36R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of blood T cell subpopulations in B-hIL36R mice

Blood routine test in B-hIL36R mice

Blood from female C57BL/6 and B-hIL36R mice (n=20, 8-week-old) was collected and analyzed for CBC. There were no differences among any measurement between C57BL/6 and B-hIL36R mice, indicating that introduction of hIL36R in place of its mouse counterpart does not change blood cell composition and morphology. Values are expressed as mean ± SEM.

Blood chemistry of B-hIL36R mice

In vivo efficacy of anti-human IL36R antibody in psoriasis model induced in B-hIL36R mice

In vivo efficacy of anti-human IL36R antibody in psoriasis model induced in B-hIL36R mice