B-hIL4/hIL4RA mice(C.B6)
| Strain Name |
C.B6-Il4tm2(IL4)Bcgen Il4ratm1(IL4RA)Bcgen/Bcgen
|
Common Name | B-hIL4/hIL4RA mice(C.B6) |
| Background | BALB/cCrSlcNifdc.C57BL/6JNifdc | Catalog number | 111889 |
|
Aliases |
BCGF-1, BCGF1, BSF-1, BSF1, IL-4 |
||
Model validation
Protein expression analysis
Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA mice(C.B6) by ELISA. Serum was collected from wild-type BALB/c mice (+/+; +/+) and homozygous B-hIL4/hIL4RA mice(C.B6) (H/H; H/H) stimulated with anti-CD3ε in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse IL4 was detectable in wild-type BALB/c mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA mice(C.B6) but not in wild-type BALB/c mice.
Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA mice(C.B6) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+; +/+) and homozygous B-hIL4/hIL4RA mice(C.B6) (H/H; H/H), and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse IL4RA was detectable in wild-type BALB/c mice. Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA mice(C.B6) but not in wild-type BALB/c mice.
Analysis of immune cells in BALF by FACS.BALF immune cells were isolated from B-hIL4/hIL4RA mice(C.B6) (n=6). The number and proportion of eosinophils were analyzed by flow cytometry under the treatment of PBS/dupilumab (in house). After treatment of dupilumab (in house), the number of CD45+ cells and eosinophils were much lower than the positive control in homozygous B-hIL4/hIL4RA mice(C.B6).
IgE production in serum of mouse asthma model.Serum was collected at the study endpoint. IgE levels responded to OVA-specific antibody and total IgE levels were analyzed. The results show that the levels of IgE in mice treated with dupilumab (in house) is much lower than that in untreated mice.
H&E staining of asthma-like model in B-hIL4/hIL4RA mice(C.B6).Lung tissues were collected at the study endpoint. H&E staining results showed that the lung tissues from B-hIL4/hIL4RA mice(C.B6) exposed to PBS aerosols did not show any inflammation. OVA exposure resulted in a significant increase in peribronchial and perivascular inflammation in B-hIL4/hIL4RA mice(C.B6). A significant reduction in eosinophils infiltration was observed in mice treated with dupilumab (in house). (a) Mucus (b)Eosinophils
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