Protein expression analysis (F3)

Strain specific LILRB3 expression analysis in transgenic B-Tg(hLILRB2-hLILRB3) mice by flow cytometry. Dendritic cells and macrophages of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB2-hLILRB3) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB3 antibody. Human LILRB3 was both detectable in dendritic cells and macrophages from B-Tg(hLILRB2-hLILRB3) mice but not wild-type mice.

In vivo efficacy of anti-human LILRB2 antibody

Antitumor activity of anti-human LILRB2 antibody in B-Tg(hLILRB2-hLILRB3) mice. (A) Anti-human LILRB2 antibody inhibited MC38 tumor growth in B-Tg(hLILRB2-hLILRB3) mice. Murine colon cancer MC38 cells were subcutaneously implanted into B-Tg(hLILRB2-hLILRB3) mice (female, 9 week-old, n=6). Mice were grouped when tumor volume reached approximately 100-150 mm³, and treated with anti-h LILRB2 antibody in panel A. (B) Body weight changes during treatment. As shown in panel A, J19.h7 (in house) was efficacious in controlling tumor growth in B-Tg(hLILRB2-hLILRB3) mice, demonstrating they provide a powerful preclinical model for in vivo evaluation of anti-human LILRB2 antibodies. Values are expressed as mean ± SEM.