| Strain Name |
C57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen
|
Common Name | B-hBTLA/hHVEM mice |
| Background | C57BL/6 | Catalog number | 121080 |
| Aliases |
BTLA, BTLA1, CD272, B and T lymphocyte associated; TNFRSF14, HVEM, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, Herpesvirus entry mediator, TNF receptor superfamily member 14 |
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mRNA expression analysis
Strain specific analysis of BTLA and HVEM gene expression in wild type (WT) mice and B-hBTLA/hHVEM mice by RT-PCR.
Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
Protein expression analysis in B cells
Strain specific BTLA expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA was detectable in WT mice (+/+). Human BTLA was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
Protein expression analysis in T cells
Strain specific HVEM expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-HVEM antibody. Mouse HVEM was detectable in WT mice (+/+). Human HVEM was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
Tumor growth curve & Body weight changes
B-hHVEM MC38 tumor cells growth of individual mice. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). As shown in panel, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
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