B-hCD3E/hCD28/hCD19 mice
| Strain Name |
C57BL/6-Cd3etm2(CD3E)Bcgen Cd28tm1(CD28)Bcgen Cd19tm4(CD19)Bcgen/Bcgen |
Common Name | B-hCD3E/hCD28/hCD19 mice |
| Background | C57BL/6 | Catalog number |
113092 |
|
Related Genes |
CD3E,CD3e molecule, IMD18, T3E, TCRE; CD28, CD28 molecule, Tp44; B4, CVID3 |
||
|
NCBI Gene ID |
12487,12478 | ||
Model validation
Description
Protein expression analysis in spleen
Frequency of leukocyte subpopulations in spleen
Frequency of leukocyte subpopulations in blood
Frequency of leukocyte subpopulations in lymph node
- CD3 and CD28 are important markers on the surface of T cells and are related to T cell activation. CD19 is a type I transmembrane protein specifically expressed in normal B cells, tumor B cells, and follicular dendritic cells, and is associated with the activation and proliferation of B cells.
- In B-hCD3E/hCD28/hCD19 mice, the extracellular region of the mouse Cd3e and Cd28 genes is replaced by the extracellular region of the human CD3E and CD28 gene, and a chimeric CD19 coding sequence containing the extracellular region of human CD19 gene is inserted into mouse Cd19 gene.
- Human CD3E, CD28 and CD19 were detectable in homozygous B-hCD3E/hCD28/hCD19 mice. Humanization of CD3E, CD28 and CD19 do not change the overall frequency or distribution of immune cell types in spleen, blood and lymph nodes.
- This product is used for pharmacodynamics and safety evaluation of B-cell lymphoma and autoimmune diseases.
Strain specific CD3E expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3E/hCD28/hCD19 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100312) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. Mouse CD3E was only detectable in wild-type C57BL/6JNifdc mice (CD3E+/+CD28+/+CD19+/+). Human CD3E was exclusively detectable in B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/+) and homozygous B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/H), but not in wild-type C57BL/6JNifdc mice.
Strain specific CD28 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3E/hCD28/hCD19 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). Protein expression was analyzed with anti-mouse CD28 antibody (Biolegend, 102105) and anti-human CD28 antibody (Biolegend, 302912) by flow cytometry. Mouse CD28 was only detectable in wild-type C57BL/6JNifdc mice (CD3E+/+CD28+/+CD19+/+). Human CD28 was exclusively detectable in B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/+) and homozygous B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/H), but not in wild-type C57BL/6JNifdc mice.
Strain specific CD19 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3E/hCD28/hCD19 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). Protein expression was analyzed with anti-mouse CD19 antibody (Biolegend, 115507) and anti-human CD19 antibody (Biolegend, 302234) by flow cytometry. Mouse CD19 was detectable in wild-type C57BL/6JNifdc mice (CD3E+/+CD28+/+CD19+/+) and B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/+). Human CD19 was detectable in B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/+) and homozygous B-hCD3E/hCD28/hCD19 mice (CD3EH/HCD28H/HCD19H/H), but not in wild-type C57BL/6JNifdc mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6JNifdc mice and homozygous B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequencies of T cell subpopulations. Percentages of T cells, B cells, NK cells, DCs, monocytes, macrophages, neutrophils, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3E/hCD28/hCD19 mice were similar to those in C57BL/6JNifdc mice, demonstrating that humanization of CD3E, CD28 and CD19 do not change the frequency or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from wild-type C57BL/6JNifdc mice and homozygous B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequencies of T cell subpopulations. Percentages of T cells, B cells, NK cells, DCs, monocytes, macrophages, neutrophils, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3E/hCD28/hCD19 mice were similar to those in C57BL/6JNifdc mice, demonstrating that humanization of CD3E, CD28 and CD19 do not change the frequency or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in lymph node by flow cytometry. Leukocytes were isolated from wild-type C57BL/6JNifdc mice and homozygous B-hCD3E/hCD28/hCD19 mice (female, 6-week-old, n=3). A. Flow cytometry analysis of the leukocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequencies of T cell subpopulations. Percentages of T cells, B cells, NK cells, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3E/hCD28/hCD19 mice were similar to those in C57BL/6JNifdc mice, demonstrating that humanization of CD3E, CD28 and CD19 do not change the frequency or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
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