Protein expression analysis

Strain specific EPCAM expression analysis in homozygous B-hCD3E/hEPCAM mice by flow cytometry. Small intestine and pancreas were collected from wild type (WT) mice (+/+; +/+) and homozygous B-hCD3E/hEPCAM mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-EPCAM antibody. Mouse EPCAM was detectable in WT mice (+/+; +/+). Human EPCAM was exclusively detectable in homozygous B-hCD3E/hEPCAM mice (H/H; H/H) but not in WT mice (+/+; +/+).

Protein expression profile of EPCAM

Immunohistochemical (IHC) Analysis of EPCAM Protein Expression in B-hCD3E and B-hCD3E/hEPCAM Mice. Tissues were collected from B-hCD3E mice and homozygous B-hCD3E/hEPCAM mice (3 females, 8 weeks old) for IHC analysis. Tissues from B-hCD3E mice were stained with an anti-mouse EPCAM antibody (abcam, ab221552), while tissues from B-hCD3E/hEPCAM mice were stained with an anti-human EPCAM antibody (abcam, ab223582). The results demonstrated that mouse EPCAM (in B-hCD3E mice) and humanized EPCAM (in B-hCD3E/hEPCAM mice) were expressed in the liver (B), lung (D), kidney (E), skin (G), stomach (H), and ileum (I). In contrast, no EPCAM expression was detected in the heart (A), spleen (C), or brain (F). These findings indicate that the expression profile of humanized EPCAM in B-hCD3E/hEPCAM mice is consistent with that of mouse EPCAM in B-hCD3E mice, confirming that humanization did not alter the tissue-specific expression pattern of EPCAM. Positive signals are indicated by arrows in the figure.