B-hDR3 mice
| Strain Name |
C57BL/6N-Tnfrsf25tm3(TNFRSF25)Bcgen/Bcgen
|
Common Name | B-hDR3 mice |
| Background | C57BL/6N | Catalog Number | 113926 |
|
Aliases |
APO-3, DDR3, DR3, GEF720, LARD, PLEKHG5, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR |
||
|
NCBI Gene ID |
8718 | ||
Model validation
Description
Targeting strategy
Protein expression analysis in T cells
Protein expression analysis in CD4+ T cells
Protein expression analysis in Treg cells
Frequency of leukocyte subpopulations in spleen
Frequency of leukocyte subpopulations in blood
Frequency of leukocyte subpopulations in lymph nodes
- DR3 is a cell surface receptor that belongs to the tumor necrosis factor receptor superfamily and plays a role in apoptosis, immune regulation, and inflammatory responses.
- The exons 1-10 of mouse Dr3 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hDR3 mice. The human DR3 expression was driven by human DR3 promoter, while mouse Dr3 gene transcription and translation will be disrupted.
- Mouse DR3 protein was exclusively detectable on T cells, CD4+ T cells and Treg cells from the spleen of wild-type C57BL/6N mice, and human DR3 protein was exclusively detectable on T cells, CD4+ T cells and Treg cells from the spleen of homozygous B-hDR3 mice. Humanization of DR3 does not change the overall frequency or distribution of immune cell types in spleen, blood and lymph nodes.
- Application: This product is used for pharmacodynamics and safety evaluation of drugs.
Gene targeting strategy for B-hDR3 mice. The exons 1-10 of mouse Dr3 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hDR3 mice. The human DR3 expression was driven by human DR3 promoter, while mouse Dr3 gene transcription and translation will be disrupted.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-mouse DR3 antibody (Biolegend, 144407) and anti-human DR3 antibody (Biolegend, 307105) by flow cytometry. Mouse DR3 was detectable in wild-type C57BL/6N mice, human DR3 was detectable in homozygous B-hDR3 mice.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-human DR3 antibody Ab1 provided by the client. Human DR3 was only detectable in homozygous B-hDR3 mice.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-mouse DR3 antibody (Biolegend, 144407) and anti-human DR3 antibody (Biolegend, 307105) by flow cytometry. Mouse DR3 was detectable in wild-type C57BL/6N mice, human DR3 was detectable in homozygous B-hDR3 mice.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-human DR3 antibody Ab1 provided by the client. Human DR3 was only detectable in homozygous B-hDR3 mice.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-mouse DR3 antibody (Biolegend, 144407) and anti-human DR3 antibody (Biolegend, 307105) by flow cytometry. Mouse DR3 was detectable in wild-type C57BL/6N mice, human DR3 was detectable in homozygous B-hDR3 mice.
Strain specific DR3 expression analysis in wild-type C57BL/6N mice and homozygous B-hDR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6N mice(+/+) and homozygous B-hDR3 mice(H/H), protein expression was analyzed with anti-human DR3 antibody Ab1 provided by the client. Human DR3 was detectable in homozygous B-hDR3 mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6N mice (+/+) (female, n=4, 7-week-old) and homozygous B-hDR3 mice(H/H) (female, n=4, 7-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hDR3 mice were similar to those in C57BL/6N mice, demonstrating that humanization of DR3 does not change the frequency or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from wild-type C57BL/6N mice (+/+) (female, n=4, 7-week-old) and homozygous B-hDR3 mice(H/H) (female, n=4, 7-week-old). A. Flow cytometry analysis of the blood was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hDR3 mice were similar to those in C57BL/6N mice, demonstrating that humanization of DR3 does not change the frequency or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in lymph nodes by flow cytometry. Lymph nodes cells were isolated from wild-type C57BL/6N mice (+/+) (female, n=4, 7-week-old) and homozygous B-hDR3 mice(H/H) (female, n=4, 7-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, CD4+ T cells, CD8+ T cells and Tregs in B-hDR3 mice were similar to those in C57BL/6N mice. Values are expressed as mean ± SEM.
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