Protein expression analysis in B cells

Strain specific SIGLEC10 expression analysis in homozygous B-hSIGLEC10 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hSIGLEC10 (H/H) mice, and analyzed by flow cytometry with species-specific SIGLEC10 antibody. Mouse SIGLEC10 was detectable in WT mice. Human SIGLEC10 was exclusively detectable in homozygous B-hSIGLEC10 but not WT mice.

Protein expression analysis in macrophages

Analysis of spleen leukocytes subpopulation in B-hSIGLEC10 mice

Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hSIGLEC10 mice(n=3, 6-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. Representative FACS plots. Single live CD45+ cells were gated for TCRb+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+, CD8+, Tregs in homozygous B-Hsiglec10 mice were similar to those in the C57BL/6 mice,  demonstrating that introduction of hSIGLEC10 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.