B-hSIRPA/hCD47,Il2rg KO mice
| Strain Name |
C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Il2rgtm1Bcgen/Bcgen
|
Common Name | B-hSIRPA/hCD47,Il2rg KO mice |
| Background | C57BL/6 | Catalog number | 130566 |
|
Aliases |
BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, IAP, MER6, OA3, IL-2RG, IMD4, P64, SCIDX, SCIDX1 | ||
|
NCBI Gene ID |
19261,16423,16186 | ||
Model validation
Protein expression analysis
Species specific SIRPα expression analysis in B-hSIRPA/hCD47,Il2rg KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Il2rg KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα antibodies. Human SIRPα was exclusively detectable in homozygous B-hSIRPA/hCD47,Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.
Species specific CD47 expression analysis in B-hSIRPA/hCD47,Il2rg KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Il2rg KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-CD47 antibodies. Mouse CD47 was only detectable in C57BL/6 mice. Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47,Il2rg KO mice but not in C57BL/6 mice. Mouse and human CD47 were not detectable on T cells when stimulated with anti-mCD3ε antibody.
Analysis of leukocyte subpopulations in spleen by FACS
Splenocytes were isolated from C57BL/6 and B-hSIRPA/hCD47,Il2rg KO mice (n=2, 5-week-old). Flow cytometry analysis of the splenocytes was performed to assess T cells and B cells. The percent of T and B cells decreased in B-hSIRPA/hCD47,Il2rg KO mice compared to C57BL/6 mice. When stimulated with anti-mCD3ε antibody, the percent of B cells also decreased in B-hSIRPA/hCD47,Il2rg KO mice compared to C57BL/6 mice, while T cells was not detectable in B-hSIRPA/hCD47,Il2rg KO mice.
Analysis of spleen leukocyte subpopulations by FACS
Splenocytes were isolated from female C57BL/6 and B-hSIRPA/hCD47,Il2rg KO mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of total T cells, CD4+ T cells and granulocytes in homozygous B-hSIRPA/hCD47,Il2rg KO mice were increased compared to those in the C57BL/6 mice, while percent of B cells, NK cells and CD8+ T cells were decreased in B-hSIRPA/hCD47,Il2rg KO mice. Results indicated that knockout of Il2rg gene has influenced the development, differentiation or distribution of these T cell subtypes in spleen.
Analysis of leukocyte subpopulations in spleen by FACS
Splenocytes were isolated from C57BL/6 and B-hSIRPA/hCD47,Il2rg KO mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells in homozygous B-hSIRPA/hCD47,Il2rg KO mice were increased compared to those in the C57BL/6 mice, while percent of CD8+ T cells and Treg cells in homozygous B-hSIRPA/hCD47 mice were decreased. Results indicated that knockout of Il2rg gene has influenced the development, differentiation or distribution of these T cell subtypes in spleen.
Analysis of T cell subpopulations in thymus
Analysis of leukocyte subpopulations in spleen by FACS
Thymocytes were isolated from C57BL/6 and B-hSIRPA/hCD47,Il2rg KO mice (n=3, 6-week-old). Flow cytometry analysis of the thymocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells in homozygous B-hSIRPA/hCD47,Il2rg KO mice was increased compared to those in the C57BL/6 mice, while percent of DP (double positive) T cells and Treg cells in homozygous B-hSIRPA/hCD47 mice were decreased. Results indicated that knockout of Il2rg gene has influenced the development and differentiation of these T cell subtypes in thymus.
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