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Animal&Cell Models

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Text

B-hCD8 mice

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Strain Name	C57BL/6-Cd8a^{tm1(CD8A)Bcgen}Cd8b^{tm1(CD8B)Bcgen}/Bcgen	Common Name	B-hCD8 mice
Background	C57BL/6	Catalog number	112811
Aliases	CD8, p32, Leu2, IMD116, CD8alpha; LY3, P37, LEU2, LYT3, Ly-3, CD8B1, CD8beta

Model validation

Protein expression analysis in blood cells

Strain specific CD8 expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD8 mice by flow cytometry. Blood were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD8 mice (H/H), respectively, and analyzed by flow cytometry with species-specific anti-hCD8a, anti-hCD8b, anti-mCD8a antibodies. hCD8a and hCD8b were exclusively detectable in homozygous B-hCD8 mice(A). mCD8a was not detectable in homozygous B-hCD8 mice(B).

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