B-hHER2 mice
| Strain Name |
C57BL/6-Erbb2tm1(ERBB2)Bcgen/Bcgen |
Common Name | B-hHER2 mice |
| Background | C57BL/6 | Catalog number | 110812 |
| Aliases | ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2 | ||
Model validation
IHC analysis of HER2 expression
Immunohistochemical (IHC) analysis of HER2 expression in homozygous B-hHER2 mice. The mammary gland, colon and stomach tissues were collected from WT and homozygous B-hHER2 mice (H/H) and analyzed by IHC with anti-Her2 antibody. HER2 was detectable in WT mice and homozygous B-hHER2 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER2 staining (brown).
Analysis of spleen leukocytes cell subpopulations in B-hHER2 mice
Analysis of spleen leukocyte subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in spleen. Values are expressed as mean ± SEM.
Analysis of lymph node leukocyte subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
Analysis of lymph node T cell subpopulations in B-hHER2 mice
Analysis of lymph node T cell subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in lymph node. Values are expressed as mean ± SEM.
Analysis of blood leukocytes cell subpopulations in B-hHER2 mice
Analysis of blood leukocyte subpopulations by FACS.Blood cells were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the blood leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Analysis of blood T cell subpopulations in B-hHER2 mice
Analysis of blood T cell subpopulations by FACS.Blood cells were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in blood. Values are expressed as mean ± SEM.
Blood routine test in B-hHER2 mice
Complete blood count (CBC). Blood from female C57BL/6 and B-hHER2 mice (n=8, 8-week-old) was collected and analyzed for CBC. There was no differences among any measurement between C57BL/6 and B-hHER2 mice, indicating that introduction of hHER2 in place of its mouse counterpart does not change blood cell composition and morphology. Values are expressed as mean ± SEM.
Blood biochemistry of B-hHER2 mice
Blood biochemistry tests of B-hHER2 mice. Serum from the C57BL/6 and B-hHER2 mice (n=8, 8-week-old) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hHER2 mice, indicating that introduction of hHER2 in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.
Different doses of anti-human HER2 antibody Ab1 were injected into B-hHER2 mice via a single intravenous injection. The body weight of B-hHER2 mice decreased in a dose-dependent manner. In the high-dose group, one mouse died. Histopathological analysis revealed no significant abnormal changes in liver, but no immature follicles were seen in ovaries, and the myeloid cells in the bone marrow increased and the red blood cell count decreased. This suggests that B-hHER2 mice can be used to assess the toxicity of anti-human HER2 antibodies. Data is obtained from a partner. Values are expressed as mean ± SEM.
Different anti-human HER2 antibodies were injected into B-hHER2 mice through a single intravenous injection. The mice in different antibody treatment groups had varying rates of weight loss. In antibody Ab1 treatment group, all mice were euthanized due to rapid weight loss. Mild toxicity was observed in the bone marrow and ovaries through histopathological analysis. This suggests that B-hHER2 mice can be used to assess the toxicity of anti-human HER2 antibodies. Data is obtained from a partner. Values are expressed as mean ± SEM.
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