Application

Expression

Expressed gene

iCre, improved cre recombinase, bacteriophage P1

Site of expression

endothelial cells, central nervous system (CNS) blood vessels

Gene editing strategy

An F2A-iCre sequence cassette was placed between the coding sequence of exon 23 and 3’UTR of the Pdgfrb gene. This strain was maintained on a C57BL/6 genetic background.

Phenotype Analysis

(B) Quantification of the number of CD31+ capillaries expressing claudin-5 or ZO-1 and the MFI of claudin-5 or ZO-1 in CD31+ capillaries showed reduction of claudin-5 in capillaries of cortex from Cd146^−/− mice compared with those from Cd146^+/+ mice at P5 and 4–6 wk. (C) Brain sections from cortex of mice at 4–6 wk were costained for CD31 (green) and PDGFRβ (red) and analyzed by LSFM after being optically cleared by using organic solvents. Representative MIPs of 40 virtual single slices from Cd146^+/+ and Cd146^−/− mice are shown. (Scale bar: 50 μm.)

(source: Chen J et al.,2017)

1. Olson LE; Soriano P. 2011. PDGFRbeta Signaling Regulates Mural Cell Plasticity and Inhibits Fat Development. Dev Cell 20(6):815-26. [PubMed: 21664579]

2. Chhabra A; Lechner AJ; Ueno M; Acharya A; Van Handel B; Wang Y; Iruela-Arispe ML; Tallquist MD; Mikkola HK. 2012. Trophoblasts regulate the placental hematopoietic niche through PDGF-B signaling. Dev Cell 22(3):651-9. [PubMed: 22387002]

3. Hellstrom M; Kal n M; Lindahl P; Abramsson A; Betsholtz C. 1999. Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development 126(14):3047-55. [PubMed: 10375497]

4. Klinghoffer RA; Mueting-Nelsen PF; Faerman A; Shani M; Soriano P. 2001. The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions. Mol Cell 7(2):343-54. [PubMed: 11239463]

5. Lindahl P; Hellstrom M; Kalen M; Karlsson L; Pekny M; Pekna M; Soriano P; Betsholtz C. 1998. Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli. Development 125(17):3313-22. [PubMed: 9693135]

6. Chen J, Luo Y, Hui H, Cai T, Huang H, Yang F, Feng J, Zhang J, Yan X. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):E7622-E7631. [PubMed: 28827364]

Pyruvate kinase M2 regulates kidney fibrosis through pericyte glycolysis during the progression from acute kidney injury to chronic kidney disease.pdf

When using the B-Pdgfrb-iCre mouse strain in a publication, please indicate the originate from Biocytogen and stock #110129 in your Materials and Methods section.