Description

The exogenous promoter and human CCL1 coding sequence were inserted into the mouse genome randomly. Human CCL1 can be secreted by B-Tg(hCCL1) MC38 cells, and drive more T cells into the tumor microenvironment in C57BL/6 mice bearing MC38 cells.

B-Tg(hCCL1) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Gene targeting strategy for B-Tg(hCCL1) MC38 cells. The exogenous promoter and human CCL1 coding sequence were inserted into the mouse genome randomly.

Protein expression analysis

CCL1 expression analysis in B-Tg(hCCL1) MC38 cells by ELISA. Human CCL1 was detected in the supernatant of B-Tg(hCCL1) MC38 cells but not wild-type MC38 cells. The 2-C12 clone of B-Tg(hCCL1) MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Analysis of tumor infiltrates lymphocytes

Data was shown as Mean±SEM, and analyzed using One way ANOVA followed  Tukey test compared with every other column (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)