Description

C5 expression analysis in B-hC5 MC38 cells by ELISA. Supernatant from wild-type MC38 and B-hC5 MC38 cultures were stained with species-specific anti-C5 antibody. Human C5 was detectable in the supernatant of B-hC5 MC38 cells but not in that of wild-type MC38 cells. The 1-G04 clone of B-hC5 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hC5 MC38 cells. B-hC5 MC38 cells (1x10⁶) and wild-type MC38 cells (1x10⁶) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. Three mice from each group were sacrificed on day 14 and the other mice were sacrificed at the endpoint. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hC5 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Protein expression analysis of C5 and C5a in serum and tumor tissues