Description 

The mouse Epcam gene was replaced by human EPCAM coding sequence in B-hEPCAM EL4 cells. Human EPCAM is highly expressed on the surface of B-hEPCAM EL4 cells.

B-hEPCAM EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Gene targeting strategy for B-hEPCAM EL4 cells. The exogenous promoter and human EPCAM coding sequence was inserted to replace part of murine exon 4 and all of exons 5~7. The insertion disrupts the endogenous murine Epcam gene, resulting in a non-functional transcript.

Protein expression analysis

EPCAM expression analysis in B-hEPCAM EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hEPCAM EL4 cultures were stained with species-specific anti-EPCAM antibody. Human EPCAM was detected on the surface of B-hEPCAM EL4 cells but not wild-type EL4 cells. The 4-B04 clone of B-hEPCAM EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Protein expression analysis of tumor cells