Description 

Gene targeting strategy for B-hGPC3 MC38 plus cells. The exogenous promoter and human GPC3 coding sequence was inserted to murine rosa26 site.

Protein expression analysis

Protein expression analysis of tumor cells

B-hGPC3 MC38 plus cells were subcutaneously transplanted into C57BL/6N mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human GPC3 expression by flow cytometry. As shown, human GPC3 was highly expressed on the surface of tumor cells. Therefore, B-hGPC3 MC38 plus cells can be used for in vivo efficacy studies of GPC3 therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hGPC3 MC38 plus cells. B-hGPC3 MC38 plus cells were subcutaneously implanted into C57BL/6 mice (male, 9-week-old) and B-hNKP46 mice (male, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hGPC3 MC38 plus cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Tumor growth curve & Body weight changes